A doctoral thesis at Karlstad University presents findings on the analysis of charged molecules using liquid chromatography, a technique widely used in the pharmaceutical industry.
Research Focus
The research, conducted by Abdul Haseeb, focuses on the fundamental interactions between charged solutes and stationary phases.
Key Findings
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Charged molecules, including newer therapeutics like oligonucleotides and peptides, exhibit complex interactions with stationary phases, leading to issues such as peak tailing, low retention, and limited separation.
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Contrary to traditional views, active sites (residual silanols) in stationary phases can improve peak shapes when controlled, rather than being removed through extensive chemical modifications.
"Active sites in stationary phases can improve peak shapes when controlled, rather than being removed."
Background
Liquid chromatography is essential for separating and measuring components in medicines, including active ingredients and excipients. The analysis of charged molecules is particularly challenging due to multiple charges in modern drugs.
Statement
According to Abdul Haseeb, a deeper mechanistic understanding of chromatographic processes is necessary to develop robust analytical methods for modern medicines, which is crucial for accurate analysis, patient safety, and regulatory approval.
"A deeper mechanistic understanding is crucial for accurate analysis, patient safety, and regulatory approval."