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Scientists Discover Protein Fibers That Enhance Bacillus Thuringiensis Pesticide Effectiveness

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Unraveling Nature's Strategy: The 'Sporesilk' Superweapon

Scientists have discovered a robust protein fiber, named 'sporesilk', that acts as a molecular net, turning a humble bacterium into a highly efficient insecticide.

(VIB/Vrije Universiteit Brussel) Researchers have revealed the secret behind the potent insecticidal power of Bacillus thuringiensis (Bt). The key is a newly identified protein fiber network, dubbed 'sporesilk', that packages the bacterium's deadly payload for maximum impact.

A Molecular Masterpiece: The Structure of Sporesilk

The study, published in Nature Communications, details a remarkable biological material.

  • A Double Helix: The fibers form a double-helical structure, incredibly thin at just eight nanometers wide.
  • Extreme Stability: These fibers are chemically crosslinked, making them exceptionally durable. They can withstand intense heat, drought, harsh chemicals, and significant mechanical stress.
  • Nature's Glue: Prof. Han Remaut (VIB-VUB) characterizes sporesilk as "one of the most robust protein materials observed in nature."

The fibers act as a molecular net that delivers both infectious spores and toxic payload simultaneously to ingested insects.
— Dr. Mike Sleutel (VIB-VUB)

How it Works: Clustering for a Lethal Strike

Bt is a widely used, eco-friendly pesticide that targets specific insect larvae while leaving humans, wildlife, and beneficial insects like bees unharmed. Its operation has two stages: first releasing toxin crystals to damage the insect's gut, then entering via spores that germinate and multiply. Until now, a key question remained: how do the spores and toxins stay together in the environment to ensure a successful infection?

This new research provides the answer:

  1. Creating Infection Units: Sporesilk clusters Bt spores and toxin crystals into compact 'infection units'.
  2. Proving the Mechanism: Deleting the gene responsible for the fibers significantly reduced this clustering and delayed insect mortality in laboratory models.
  3. Reversing the Effect: Critically, adding purified fibers back or engineering their production restored clustering and increased killing efficiency, confirming their role.

From Biopesticide to Biomaterial: Future Applications

This discovery has significant implications for both agriculture and materials science.

  • Better Biopesticides: The findings could lead to the development of more potent and reliable biopesticides, offering stronger crop protection while maintaining a high safety standard.
  • Inspiring New Materials: The extreme durability and the fact that sporesilk is self-assembling could inspire the creation of novel biomaterials for use in biotechnology and engineering.